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| 高铁培养环境对小鼠前成骨细胞MC3T3-E1增殖、分化的影响 |
| Effect of excess iron culturing on the proliferation and differentiation of mouse MC3T3-E1 preosteoblasts |
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| DOI：10.3969/j.issn.1006-7108.2013.10.003 |
| 中文关键词: 铁离子 成骨细胞 细胞分化 |
| 英文关键词:Iron ion Osteoblast Differentiation |
| 基金项目:国家自然科学基金(81273090)；江苏省自然科学基金(BK2012608)；苏州市科技基础设施建设
计划之高技术研究重点实验室资助项目(SZS2012208)；苏州市应用基础研究计划之医疗卫生部分资助项目
(SYS201002) |
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| 中文摘要: |
| 目的 观察高铁培养环境下小鼠前成骨样细胞MC3T3-E1增殖、分化指标的变化趋势，探讨铁离子对
成骨细胞增殖、分化的影响。方法 小鼠前成骨样细胞MC3T3-E1在37℃条件下体外培养，在10mmol/Lβ-甘
油磷酸和50μg /mL抗坏血酸的诱导分化的作用下，分化为成骨细胞，同时用不同浓度(50、100、
200μmol/L)枸橼酸铁铵(FAC)干预，用MTT法检测细胞的增殖活性，RT-PCR法检测成骨细胞分化基因成骨
相关转录因子(Runx2) 、锌指结构转录因子(Osterix) 、骨唾液酸蛋白(BSP)和骨钙素(OC)的表达，碱性
磷酸酶(ALP)活性试剂盒检测细胞碱性磷酸酶活性。结果 MC3T3-E1细胞的增殖活性、成骨分化相关基因的
表达以及ALP水平随FAC干预浓度的增加呈剂量依赖性降低(P<0.05)。结论 高铁培养环境可明显抑制小鼠
前成骨样细胞MC3T3-E1的增殖和分化。 |
| 英文摘要: |
| Objective To observe the changing pattern of proliferative and differentiatial
indexes of mouse MC3T3-E1 preosteoblasts in the culture with excess iron, and to investigate
the effect of iron on the proliferation and differentiation of osteoblasts. Methods Mouse
MC3T3-E1 preosteoblasts were cultured at 37oC in vitro. Under the induction of 10mmol/l β-
glycerophosphate and 50μg/ml L-ascorbic acid, MC3T3-E1 preosteoblasts differentiated into
osteoblasts. Meanwhile, different concentrations (50, 100, and 200 μmol/l) of FAC were
additioned into the medium for intervention. Proliferation of MC3T3-E1 cells was detected
using MTT method. The mRNA expressions of Runx2, Osterix, bone sialoprotein (BSP), and
osteocalcin (OC) were detected using RT-PCR. ALP activity was measured using ALP viability
kit. Results The proliferation of MC3T3-E1 cells, the mRNA expressions of Runx2, Osterix,
BSP, and OC, and ALP activity decreased along with the increase of FAC concentration,
showing a dose-dependent manner (p<0.05). Conclusion Excess iron culturing can significantly
inhibit the proliferation and differentiation of mouse MC3T3-E1 preosteoblasts. |
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