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| 骨膜联合骨髓间充质干细胞/乳酸乙醇酸共聚物修复兔大段尺骨缺损的研究 |
| Study on repairing of the ulna large segmental defect with combination of the periosteum and bone marrow mesenchymal stem cells in rabbits |
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| DOI：10.3969/j.issn.1006.7108.2017.07.006 |
| 中文关键词: 骨髓间充质干细胞 骨膜 骨缺损 骨再生 动物实验 |
| 英文关键词:Mesenchymal stem cells Periosteum Bone defects Regeneration Animal experimentation |
| 基金项目:皖南医学院中青年自然基金（WK2014F14) |
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| 中文摘要: |
| 目的 研究骨髓间充质干细胞（mesenchymal stem cells，MSCs)/乳酸乙醇酸共聚物（poly lactic-co-glycolic acid，PLGA)联合骨膜修复大段兔尺骨骨缺损的研究。方法 20只成年新西兰大白兔随机分成4组：PLGA组、PLGA/骨膜组、MSCs/PLGA 组和MSCs/PLGA/骨膜组。所有动物构建双侧尺骨中段15 mm的长度节段性骨缺损，随后PLGA组、PLGA/骨膜组、MSCs/ PLGA组和MSCs /PLGA/骨膜组分别植人PLGA、PLGA/骨膜、MSCs/PLGA、MSCs/PLGA/骨膜生物材料。正常环境下饲养，在术后6 w和12 w时，对缺损区域进行大体观察、影像学及组织学研究。结果 研究结果表明相同时间点MSCs/PLGA/骨膜组缺损修复效果明显优于其他组，MSCs/PLGA/骨膜植入组有最高的X线评分及缺损区最多骨缺损被修复（P < 0. 05)。PLGA/骨膜、MSCs/PLGA组在X线评分和骨量上差异无统计学意义（P > 0. 05 )，但均明显高于PLGA组（P < 0. 05 )。结论 MSCs/ PLGA联合骨膜可以较好地修复大段兔尺骨骨缺损。 |
| 英文摘要: |
| Objective To investigate the bone marrow mesenchymal stem cells/periosteum coated (lactic acid-glycolic acid, PLGA) in promoting the healing of large bone defect in the ulna in rabbits. Methods Twenty adult New Zealand rabbits were rand omly divided to PLGA group, PLGA/periosteum group, PLGA/MSCs group, and PLGA/M SC s/periosteum group. Bone defect of 15 mm segment in length was established in the middle of the ulnar in rabbits of each group. PLGA, PLGA/periosteum, PLGA/MSCs, and PLGA/MSCs/periosteum were implanted in PLGA group, PLGA/periosteum group, PLGA/MSCs group, and PLGA/M SC s/periosteum group, respectively. After 6 and 12 weeks, the samples were evaluated with radiological and histological analysis. Results Compared with other treatment groups at each time point, a better bone quality, higher X-ray scores, and more bone mass were observed in PLGA/MSCs/periosteum group (P < 0. 05 ) . There was no significant difference ( P > 0. 05 ) between PLGA/periosteum group and PLGA/MSCs group in the X-ray score and bone mass，but the X-ray score and bone mass in PLGA/ periosteum group and PLGA/MSCs group were significantly higher than those in group PLGA (P < 0. 05). Conclusion The combined use of bone marrow mesenchymal stem cells and periosteum-coated PLGA significantly promotes the healing of large ulnar bone defect in rabbits. |
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