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| 甜菜碱通过IGF-lR/β-catenin信号通路介导对2型糖尿病大鼠骨流失的保护作用 |
| Protective effect of betaine on bone loss in the type 2 diabetic rats is mediated by IGF-1R/ β- catenin signal pathway |
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| DOI：10.3969/j.issn.1006-7108.2021.01.018 |
| 中文关键词: 甜菜碱 骨量流失 IGF-lR/β-Catenin信号通路 骨密度 骨强度 |
| 英文关键词:betaine bone loss IGF-1R/β-catenin signaling pathway bone mineral density bone strength |
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| 中文摘要: |
| 目的 探讨甜菜碱（betaine，TCJ)对2型糖尿病大鼠骨量流失的影响，并探讨可能的机制。方法30只大鼠被随机分为对照组（CON)、模型组（MOD)及甜菜碱组（TCJ)，每组10只，其中对MOD组和TCJ组建立2型糖尿病模型。随后TCJ组大鼠每天接受50 mg/kg甜菜碱治疗12周，待治疗结束后通过Micro-CT检测、HE染色切片以及蛋白质印迹检测。结果 治疗12周后，与MOD组相比，三点弯曲试验、Micro-CT和HE染色切片结果显示TCJ组的大鼠骨小梁数量、骨强度和骨密度得到明显改善（P<0.05)。TCJ组大鼠最大载荷和弹性模量、骨密度（bone mineral density, BMD)、TV/BV、Tb.N、Tb.Th和Tb.Sp较 MOD 组明显改善（P<0. 05)。和 MOD 组比较，TCJ 组 β-catenin 表达水平明显上调，而 p-IGF-lR、p-GSK-3β、p-β-catenin、IGF-1R 和GSK-3β表达水平显著下调，比较差异有统计学意义（P<0.05)。结论 甜菜碱通过IGF-lR/β-catenin信号通路介导对2型糖尿病大鼠骨流失起到保护作用。 |
| 英文摘要: |
| Objective To investigate the effect of betaine (TCJ) on bone loss in type 2 diabetic rats and explore the possible mechanisms. Methods Thirty rats were randomly divided into a control group (CON)，a model group (MOD)，and a betaine group (TCJ)，10 in each group. Among them，type 2 diabetes models were established for MOD and TCJ group. Then TCJ group rats received 50 mg per day / kg betaine treatment for 12 weeks ；after the treatment, they were detected by Micro-CT, HE stained sections and Western blot. Results After 12 weeks of treatment, compared with the MOD group, the result of the three-point bending test, Micro-CT and HE staining showed that the number of trabeculae, bone strength and bone density of the TCJ group were significantly improved(P<0.05) .The maximum load and elastic modulus, BMD, TV/BV, Tb.N, Tb.Th and Tb.Sp of the TCJ group were significantly improved compared with the MOD group (P<0. 05). Compared with the MOD group, the expression level of β-catenin in the TCJ group was significantly increased, while the expression levels of p-IGF-lR, p-GSK-3β, p-β-catenin,IGF-1R and GSK-3β were significantly reduced(P<0. 05). Conclusion Betaine mediates the protective effect on bone loss in type 2 diabetic rats through IGF-1R/β-catenin signaling pathway. |
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