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| 肉苁蓉治疗慢性肾脏病和骨质疏松症的潜在双效成分及作用机制 |
| Potential dual-effect components and mechanism of Cistanche Herba in the treatment of chronic kidney disease and osteoporosis |
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| DOI：10.3969/j.issn.1006.7108.2022.04.014 |
| 中文关键词: 肉苁蓉 网络药理学 分子对接 慢性肾脏病 骨质疏松症 |
| 英文关键词:Cistanches Herba network pharmacology molecular docking chronic kidney disease osteoporosis |
| 基金项目:广东省中医药局单方验方整理研究项目（20183006），广东省中医药局面上项目（20211205） |
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| 中文摘要: |
| 目的 运用网络药理学和分子对接技术探讨肉苁蓉治疗慢性肾脏病合并骨质疏松症(CKD-OP)的潜在双效成分及作用机制。方法 通过多个数据库和文献检索筛选出肉苁蓉的活性成分及其对应靶点与慢性肾脏病(chronic kidney disease，CKD)和骨质疏松症(osteoporosis，OP)疾病相关靶点的交集，于STRING数据库构建PPI网络，经CytoHubba插件筛选核心靶点后在DAVID数据库进行GO功能和KEGG通路富集分析，于Cytoscape构建活性成分-核心靶点-信号通路网络后获得关键成分、关键靶点和关键信号通路，最后在AutoDockTools进行分子对接验证。结果 得到肉苁蓉治疗CKD-OP关键成分3个(槲皮素、β-谷甾醇、红景天苷)，关键靶点6个(TNF、AKT1、MAPK1、JUN、RELA和PTGS2)，关键信号通路3条(TNF 、NF-κB 和Oxytocin 信号通路)。结论 初步揭示肉苁蓉通过“多成分-多靶点-多途径”的特点治疗CKD-OP的潜在分子药理机制，为中药现代化和CKD-OP的治疗提供了理论依据和思路。 |
| 英文摘要: |
| Objective To explore the potential dual-effect components and mechanism of Cistanches Herba in the treatment of osteoporosis with chronic kidney disease by network pharmacology and molecular docking technology. Methods Through multiple databases and literature searches, the intersection of the active ingredients of Cistanche and their corresponding targets with Chronic Kidney Disease (CKD) and Osteoporosis (OP) related disease targets was screened out,and then, construct a PPI network in the STRING database, screen the core targets with the CytoHubba plug-in, and perform GO ontology and KEGG pathway enrichment analysis in the DAVID database. After constructing the active ingredient-core target-signaling pathway network in Cytoscape, the key components, key targets and Key signal pathways, and finally molecular docking verification in AutoDockTools. Results Obtained 3 key components of Cistanche for the treatment of CKD-OP (quercetin, β-sitosterol, salidroside), 6 key targets (AKT1, TNF, MAPK1, JUN, RELA and PTGS2), 3 key signal pathways (TNF , NF-κB and Oxytocin signaling pathways). Conclusion The preliminary discovery of the potential molecular pharmacological mechanism of Cistanche cistanche in the specific treatment of CKD-OP through "multi-component-multi-target-multi-pathway" provides a theoretical basis and ideas for the modernization of traditional Chinese medicine and the treatment of CKD-OP. |
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