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| LncRNA NUTM2A-AS1调控miR-183-5p/TGFα影响骨关节炎软骨细胞增殖、凋亡的研究 |
| LncRNA NUTM2A-AS1 regulates the molecular mechanism of miR-183-5p/TGFα on the proliferation, apoptosis of osteoarthritis chondrocytes |
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| DOI：10.3969/j.issn.1006-7108.2022.06.003 |
| 中文关键词: LncRNA NUTM2A-AS1 miR-183-5p TGFα 关节软骨细胞 凋亡 |
| 英文关键词:LncRNA NUTM2A-AS1 miR-183-5p TGFα chondrocytes apoptosis |
| 基金项目:洛阳市科技局医疗卫生重点项目（2101027A）；河南省医学科技攻关计划联合共建项目（LHGJ20191226）；陕西省自然科学基础研究计划资助项目(2021JZ-59 )；西安市卫生健康委员会项目（2020ms06） |
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| 中文摘要: |
| 目的 探讨LncRNA NUTM2A-AS1对白细胞介素-1β（IL-1β）诱导的软骨细胞损伤的影响及分子机制。方法 将软骨细胞随机分为对照组、模型组（5 μg/L的IL-1β）、miR-183-5p+模型组、miR-NC+模型组、si-LncRNA NUTM2A-AS1+模型组、si-TGFα+模型组、si-NC+模型组、pcDNA-TGFα+si-LncRNA NUTM2A-AS1+模型组、pcDNA+si-LncRNA NUTM2A-AS1+模型组；运用实时荧光定量PCR（RT-qPCR）检测LncRNA NUTM2A-AS1、miR-183-5p和TGFα mRNA的表达水平；运用细胞计数试剂盒8（CCK-8）检测细胞活性；运用流式细胞术检测软骨细胞凋亡情况；通过酶联免疫吸附法（ELISA）检测TNF-α、IL-6水平；通过双荧光素酶报告实验检测NUTM2A-AS1、miR-183-5p、TGFα之间的靶向关系。结果 IL-1β诱导的软骨细胞中LncRNA NUTM2A-AS1和TGFα表达升高，miR-183-5p表达降低，软骨细胞活性降低，而凋亡率升高，TNF-α、IL-6水平升高。低表达LncRNA NUTM2A-AS1、低表达TGFα或过表达miR-183-5p后可促进细胞增殖及抑制细胞凋亡、炎症反应。结论 低表达LncRNA NUTM2A-AS1通过调控miR-183-5p/TGFα抑制IL-1β诱导的软骨细胞凋亡及炎症反应，促进细胞存活。 |
| 英文摘要: |
| Objective To explore the effect of LncRNA NUTM2A-AS1 on interleukin-1β (IL-1β)-induced chondrocyte damage and its molecular mechanism. Methods Chondrocytes were randomly divided into control group, model group (5 μg/L IL-1β), miR-183-5p+model group, miR-NC+model group, si-LncRNA NUTM2A-AS1+model group, si-TGFα+model group, si-NC+model group, pcDNA-TGFα+si-LncRNA NUTM2A-AS1+model group, pcDNA+si-LncRNA NUTM2A-AS1+model group; real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression levels of LncRNA NUTM2A-AS1, miR-183-5p and TGFα mRNA;cell counting kit 8 (CCK-8) was used to detect cell viability; flow cytometry was used to detect chondrocyte apoptosis; enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of TNF-α and IL-6; dual luciferase reporter experiment was used to detect the targeting relationship between NUTM2A-AS1, miR-183-5p, and TGFα. Results In IL-1β-induced chondrocytes, the expressions of LncRNA NUTM2A-AS1 and TGFα were increased, the expression of miR-183-5p was decreased, the activity of chondrocytes was decreased, the apoptosis rate was increased, and the levels of TNF-α and IL-6 were increased. Low expression of LncRNA NUTM2A-AS1, low expression of TGFα or overexpression of miR-183-5p could promote cell proliferation and inhibit apoptosis and inflammatory responses. Conclusions The low expression of LncRNA NUTM2A-AS1 can inhibit IL-1β-induced chondrocyte apoptosis and inflammation by regulating miR-183-5p/TGFα, and promote cell survival. |
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