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| 肠道菌群介导贞术调脂胶囊抑制去势小鼠骨丢失的机制研究 |
| Study on the Mechanism of FTZ on bone loss in ovariectomized mice via gut microbiota |
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| DOI：10.3969/j.issn.1006-7108.2022.12.017 |
| 中文关键词: 骨质疏松症 肠道菌群 去卵巢小鼠 贞术调脂胶囊 |
| 英文关键词:osteoporosis gut microbiota ovariectomized mice ZhenzhuTiaozhi Capsule |
| 基金项目:广东省云浮市科技计划项目（2020A090402）；广东省中医药局科研项目（20192044）；广东省中医药局科研项目（20222120）；国家自然科学基金项目（青年科学基金）（81704098） |
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| 中文摘要: |
| 目的 基于肠道菌群的变化，探讨贞术调脂胶囊（FTZ）抑制去卵巢（OVX）小鼠骨丢失的作用机制。方法选取18只11周C57BL6/J雌性小鼠，平均分为假手术组（SHAM组）、OVX组和FTZ组。FTZ组在OVX基础上给予FTZ[1.55 g/(kg·d）]灌胃，SHAM组和OVX组以等剂量0.9%氯化钠溶液灌胃，12周后收集小鼠左侧股骨、血清和粪便标本。左侧股骨行Micro-CT扫描；采用ELISA法检测血清中TNF-α、IL-10、IL-6、LPS和TRACP5b的含量；末次灌胃结束24 h后收集小鼠粪便，提取并检测粪菌总DNA，利用16S rRNA高通量肠菌基因测序技术，对标本中的细菌16S rRNA 基因V3-V4可变区进行定性分析和OUT分析。结果 与SHAM组相比，OVX组BV/TV、Tb.N、BMD和IL-10均显著性降低(P＜0. 05)，Tb.Sp、TNF-α、IL-6、LPS和TRACP5b显著性增高（P＜0. 05）；与OVX组相比，FTZ组Tb.N和IL-10显著性增高（P＜0. 05），Tb.Sp、TNF-α、IL-6和TRACP5b显著性降低（P＜0. 05）。在门水平，OVX组Firmicutes/Bacteroidota（F/B）率较SHAM组有增高趋势（P=0.310），但无显著性差异，而与OVX组相比，FTZ组F/B率显著性降低（P=0.002）。在属水平，与SHAM组相比，Faecalibaculum和Coriobacteriaceae_UCG-002丰度在OVX组中显著减少（P=0.030，P=0.005），Lachnoclostridium丰度在OVX组中显著增加（P=0.037），而与OVX组相比，FTZ组Faecalibaculum和Coriobacteriaceae_UCG-002丰度显著增加（P =0.045，P=0.005），Lachnoclostridium丰度显著减少（P=0.013）。结论 FTZ能够延缓去势小鼠骨量丢失，其机制可能是通过调节肠道菌群中Faecalibaculum、Coriobacteriaceae_UCG-002和Lachnoclostridium丰度实现的，且同时抑制炎性反应。 |
| 英文摘要: |
| Objective To investigate the regulatory mechanism of FTZ inhibiting bone loss in ovariectomized (OVX) mice based on the changes of gut microbiota. Methods Eighteen 11-week-old C57BL6/J female mice were randomly divided into three groups: SHAM group (n=6) and OVX group (n=6) received an equal dose of 0.9% NaCl in the same manner; FTZ group (n=6) received FTZ at a dose of 1.55 g/kg/d via oral gavage. After 12 weeks of intervention, the outcomes were changes in bone microstructures as measured by Micro-CT scanning analysis, serum parameters (TNF-α, IL-10, IL-6, LPS and TRACP5b) and gut microbiota were measured by ELISA and 16S rRNA sequencing respectively. Results Compared with SHAM group, BV/TV, Tb.N, BMD and concentration of IL-10 were significantly decreased (P<0.05); Tb.Sp and concentration of TNF-α, IL-6, LPS and TRACP5b were significantly increased (P<0.05) in OVX group. The Tb.N and IL-10 were significantly increased (P<0.05), Tb.Sp, TNF-α, IL-6 and TRACP5b were significantly decreased in FTZ group compared with OVX group (P<0.05). At the phylum level, there was an increasing trend in F/B ratio (P =0.310) in OVX group compared with SHAM group. F/B ratio was significantly lower in FTZ group compared with OVX group (P=0.002). At the genus level, Faecalibaculum and Coriobacteriaceae_UCG-002 were significantly decreased (P=0.030, P=0.005) and Lachnoclostridium was significantly increased (P=0.037) in OVX group compared with SHAM group. Faecalibaculumand and Coriobacteriaceae_UCG-002 were significantly increased (P=0.045, P=0.005) and Lachnoclostridium was significantly decreased (P=0.013) in FTZ group. Conclusions FTZ can alleviate bone loss in OVX mice may be achieved by regulating the abundance of Faecalibaculum, Coriobacteriaceae_UCG-002 and Lachnoclostridium, meanwhile ameliorating the inflammatory response. |
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