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| 新型成骨多肽治疗去卵巢大鼠骨质疏松的作用研究 |
| Effect of the novel osteogenic polypeptide on osteoporosis in ovariectomized rats |
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| DOI：10.3969/j.issn.1006-7108.2025.09.004 |
| 中文关键词: 成骨多肽 B19 去卵巢 绝经后骨质疏松 骨密度 骨流失 |
| 英文关键词:osteogenic polypeptide B19 ovariectomy postmenopausal osteoporosis bone mineral density bone loss |
| 基金项目:甘肃省科技重大专项（24ZDFA008）；甘肃省自然科学基金（22JR11RA013）；兰州市科技计划项目（2023-1-8，2023-ZD-183） |
| 作者 | 单位 | | 王新扬1,2,3 安梓栋2,3 庞永杰2,3 武艺2,3 王立强2,3 魏振龙2,3 高玉海2,3 陈克明1,2,3* | 1.甘肃农业大学生命科学技术学院，甘肃 兰州 730070
2.中国人民解放军联勤保障部队第九四〇医院基础医学实验室，甘肃 兰州730050
3.甘肃省干细胞与基因药物重点实验室，甘肃 兰州 730050 |
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| 中文摘要: |
| 目的 研究一种新型成骨多肽B19治疗去卵巢大鼠骨质疏松的作用。方法 将3月龄体质量（240±5）g雌性SD大鼠40只，随机分为假手术组（Sham）、去卵巢组（OVX）、炔雌醇组（EE）和B19组。除Sham组外，建立去卵巢模型，每2周做1次全身骨密度检查，8周后OVX组骨密度较Sham组差异显著，开始给药。EE组口服给药剂量为100 μg/(kg·d)，B19组腹腔注射剂量为25 mg/(kg·d)，Sham组与OVX组注射等体积生理盐水。每周称重，至第6周全身骨密度出现差异，取血处死，收集主要脏器、股骨、胫骨与椎骨，做病理学分析及Mirco-CT、离体骨密度、生物力学和血清骨代谢指标检测。结果 实验期间大鼠体重差异无统计学意义；主要脏器中子宫差异存在统计学意义，与Sham组相比，OVX组脏器系数下降明显，与OVX组相比，EE组与B19组脏器系数显著提高；与OVX组相比，EE组与B19组骨密度及生物力学参数明显增高；Mirco-CT结果表明，与OVX组相比，EE组与B19组骨小梁数量（Tb.N）、骨小梁厚度（Tb.Th）与骨体积分数（BV/TV）均显著提升，骨小梁分离度（Tb.Sp）显著下降；血清结果显示，EE组与B19组血清骨形成标志物PINP、BALP水平明显高于OVX组，骨吸收标志物TRACP、CTX-I水平明显降低。结论 多肽B19可能通过促进骨形成，抑制骨吸收改善骨微环境，从而达到治疗骨质疏松的效果。 |
| 英文摘要: |
| Objective To study the effect of a novel osteogenic polypeptide B19 on osteoporosis in ovariectomized rats. Methods Forty 3-month-old female SD rats with a body weight of (240±5) g were randomly divided into sham surgery group (Sham), ovariectomy group (OVX), ethinylestradiol group (EE), and B19 group. Except for Sham group, an ovariectomized model was established. The whole body bone mineral density was examined every 2 weeks. After 8 weeks, there was a significant difference in bone mineral density between the OVX group and the Sham group, and medication was started. Rats in EE group received oral dose of EE 100 μg/(kg·d). Rats in B19 group received intraperitoneal injection of B19 25 mg/(kg·d). Rats in Sham group and OVX group received the same volume of normal saline. Body weight was examined every week. There was a difference in bone mineral density of the whole body by week 6. Rats were sacrificed and serum was collected. The major organs, femur, tibia, and vertebrae were collected for pathological analysis. Micro-CT, ex vivo bone mineral density, biomechanics, and serum bone metabolism indicators were detected. Results The body weight of rats during the experiment was not statistically significant. There was a statistical difference in the uterus among the main organs. Compared to that in Sham group, the organ coefficient in OVX group decreased significantly. Compared to that in OVX group, the organ coefficient in EE group and B19 group increased significantly. Compared to those in OVX group, bone mineral density and biomechanical parameters in EE group and B19 group were significantly higher. Mirco-CT results showed that in B19 group, the number of trabecular bones (Tb.N), trabecular bone thickness (Tb.Th), and bone volume fraction (BV/TV) in EE group increased significantly compared to those in OVX group, and the separation of trabecular bones (Tb.Sp) decreased significantly. Serum results showed that the contents of serum bone formation markers PINP and BALP in EE group and B19 group were significantly higher than those in OVX group, and the contents of bone resorption markers TRACP and CTX-I were significantly lower in EE group and B19 group. Conclusion Polypeptide B19 may improve bone microenvironment by promoting bone formation, inhibiting bone resorption, and thus achieving the effect of treating osteoporosis. |
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