中国骨质疏松杂志

温针灸腰背部脊穴和结筋病灶对去卵巢骨质疏松小鼠股骨骨密度和骨代谢调节

Effects of warm acupuncture at lumbar-dorsal spinal acupoints and lesion of tendon nodes on femoral bone mineral density and bone metabolism in ovariectomized osteoporotic mice

DOI：10.3969/j.issn.1006-7108.2026.03.006

中文关键词: 温针灸腰背部脊穴结筋病灶点骨质疏松症 PI3K/AKT/mTOR信号通路

英文关键词:warm acupuncture lumbar-dorsal spinal acupoints lesion points of tendon nodes postmenopausal osteoporosis PI3K/AKT/mTOR signaling pathway

基金项目:云南省基础研究计划项目（202101AZ070001-134）

作者	单位
王艺婷1 李绍荣2* 陈奇刚1 陈艳林1 张海金1 钱超3	1. 昆明市中医医院康复科,云南昆明 650000 2. 昆明市中医医院针灸科,云南昆明 650000 3. 昆明市中医医院骨伤科,云南昆明 650000

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中文摘要:

目的通过腰背部脊穴和结筋病灶点的单独或联合刺激，探究温针灸对绝经后骨质疏松症（postmenopausal osteoporosis，PMOP）的作用。方法将30只C57BL/6小鼠分为5组：假手术组、模型组、温针灸Ⅰ组、温针灸Ⅱ组和温针灸Ⅲ组，每组6只。使用双侧卵巢摘除术构建PMOP模型并进行温针灸治疗4周。Micro-CT检测股骨骨密度（BMD）；HE和Masson染色观察股骨病理变化和胶原纤维新生情况。ELISA检测血清中雌二醇（E2）、骨钙素（BGP）、Ⅰ型胶原交联C末端肽（ICTP）、抗酒石酸酸性磷酸酶（TRACP）和骨碱性磷酸酶（BALP）水平；Western blot检测股骨中PI3K、AKT和mTOR蛋白表达水平。结果与假手术组比较，模型组小鼠BMD、骨小梁数量、E2和BGP水平下降（P < 0.05），而ICTP、TRACP和BALP水平上升（P < 0.05）。温针灸治疗后BMD、骨小梁数量和胶原纤维增加。在3个治疗组中，温针灸Ⅲ组对E2、ICTP和BGP的调节作用最强（P < 0.05）。Western blot结果显示，模型组PI3K、AKT和mTOR的蛋白表达水平低于假手术组（P < 0.001），温针灸治疗组则高于模型组（P < 0.01），且温针灸Ⅲ组对PI3K、AKT和mTOR蛋白表达水平的上调作用最显著（P < 0.01）。结论温针灸通过对腰背部脊穴和结筋病灶点的联合刺激可促进BMD增加、骨小梁和胶原纤维新生并调节骨代谢，从而改善PMOP，其机制是激活PI3K/AKT/mTOR信号通路。

英文摘要:

Objective To investigate the effects of Warm acupuncture on postmenopausal osteoporosis (PMOP) through individual or combined stimulation of lumbar-dorsal spinal acupoints and lesion points of tendon nodes. Methods Thirty C57BL/6 mice were randomly divided into five groups (n=6 per group): sham-operated group, model group, warm acupuncture I group, warm acupuncture II group, and warm acupuncture III group. PMOP models were established via bilateral ovariectomy and treated with warm acupuncture for 4 weeks. Femoral bone mineral density (BMD) was analyzed using micro-CT. Pathological changes and collagen fiber regeneration in femoral tissues were observed via hematoxylin-eosin (HE) and Masson staining. Serum concentrations of estradiol (E2), osteocalcin (BGP), type I collagen cross-linked C-terminal telopeptide (ICTP), tartrate-resistant acid phosphatase (TRACP), and bone alkaline phosphatase (BALP) were measured by ELISA. Protein expression levels of PI3K, AKT, and mTOR in femoral tissues were detected via Western blot. Results Compared with the sham-operated group, the model group exhibited decreased BMD, trabecular bone volume, and serum E2 and BGP levels (P < 0.05), while ICTP, TRACP, and BALP levels were increased (P < 0.05). Warm acupuncture treatment increased BMD, trabecular bone volume, and collagen fiber density. Among the three warm acupuncture groups, warm acupuncture III demonstrated the strongest regulatory effects on E2, ICTP, and BGP (P < 0.05). Western blot results showed that PI3K, AKT, and mTOR protein expression levels in the model group were lower than those in the sham-operated group (P < 0.001), whereas warm acupuncture-treated groups exhibited higher expression levels than the model group (P < 0.01), with warm acupuncture III showing the most pronounced upregulation (P < 0.01). Conclusion Combined stimulation of lumbar-dorsal spinal acupoints and lesion points of tendon nodes through warm acupuncture promotes BMD elevation, trabecular bone and collagen fiber regeneration, and bone metabolism regulation, thereby ameliorating PMOP. The underlying mechanism involves activation of the PI3K/AKT/mTOR signaling pathway.

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