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| 针刺调控雌激素受体水平治疗绝经后骨质疏松症的机制 |
| Mechanism of acupuncture in regulating estrogen receptor levels for the treatment of postmenopausal osteoporosis |
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| DOI：10.3969/j.issn.1006-7108.2026.05.007 |
| 中文关键词: 针刺 绝经后骨质疏松症 雌激素受体α 雌激素受体β |
| 英文关键词:acupuncture postmenopausal osteoporosis estrogen receptor α estrogen receptor β |
| 基金项目:云南省基础研究计划面上项目（202501AT070423） |
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| 中文摘要: |
| 目的 基于ERα、ERβ调控OPG/RANK/RANKL通路探讨针刺防治绝经后骨质疏松症（postmenopausal osteoporosis，PMOP）的作用机制。方法 选取60只SD雌性大鼠随机分为正常对照组（Control组)、模型组，采用去势术建立PMOP动物模型，验证造模成功后将造模后的大鼠随机分为模型组（Model组）、健脾益肾强骨针法组（Acu组）、雌二醇组（E2组）、健脾益肾强骨针法+雌二醇组（Acu-E2组），分别按照相应的针刺、E2药物和针刺联合药物治疗干预6个月。Micro-CT断层扫描感兴趣区域图像，分析各组大鼠左侧股骨骨密度（BMD）、骨体积分数（BV/TV）、骨小梁厚度（Tb.Th）、骨小梁数（Tb.N）、骨小梁分离度（Tb.Sp）；HE染色法观察大鼠左侧股骨组织形态变化；酶联免疫吸附法(ELISA)检测大鼠血清中E2、卵泡刺激素（FSH）、促性腺激素释放激素（GnRH）、抗酒石酸酸性磷酸酶（TRACP）、Ⅰ型胶原C端肽（CTX-Ⅰ）的水平；逆转录聚合酶链反应(RT-PCR)测定下丘脑及骨髓细胞ERα、ERβ、OPG、RANK和RANKL mRNA水平；免疫印记法(Western blot)测定右侧股骨组织ERα、ERβ蛋白表达水平。结果 与Model组比较，Acu组、E2组和Acu-E2组能显著改善大鼠股骨组织的BMD和骨微结构参数(P＜0.05)以及其病理变化；同时降低大鼠血清TRACP和CTX-Ⅰ含量，减少血清FSH、GnRH和增加E2含量(P＜0.05)；并且增加大鼠下丘脑和骨髓细胞ERα、ERβ和OPGmRNA表达水平，降低RANK和RANKL mRNA的表达水平。结论 针刺可有效防治PMOP，其机制可能与通过激活OPG/RANK/RANKL通路，对下丘脑-垂体-骨骼轴起整体调节作用，增加雌激素与受体结合的生物学效应相关。 |
| 英文摘要: |
| Objective Based on ERα and ERβregulation of OPG/RANK/RANKL pathway to explore the mechanism of acupuncture in preventing and treating postmenopausal osteopor-osis (PMOP). Methods Sixty female SD rats were randomly divided into a normal control group (Control group) and a model group. PMOP animal models were established using ovariectomy, and successful modeling was confirmed. The ovariectomized rats were then randomly divided into the model group (Model group）, the spleen-kidney strengthening and bone enhancing acupuncture group (Acu group), the estradiol group (E2 group), and the acupuncture combined with estradiol group (Acu-E2 group). These groups received corresponding acupuncture, E2 medication, or combined treatment for six months. Micro-CT was used to scan images of the region of interest, and parameters including bone mineral den-sity (BMD), bone volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular numb-er(Tb.N), and trabecular separation (Tb.Sp) of the left femur were analyzed. Hematoxyline-osin (HE)staining was employed to observe morphological changes in the left femoral tiss-ue. Enzyme-linked immunosorbent assay (ELISA)was used to measure serum levels of est-radiol (E2),follicle-stimulating hormone (FSH),gonadotropin-releasinghormone(GnRH), tartrate-resistant acid phosphatase (TRACP), and C-terminal telopeptide of type I collagen (CTX-I). Reverse transcription-polymerase chain reaction (RT-PCR) was performed to determine m-RNA levels of ERα, ERβ, OPG, RANK, and RANKL in the hypothalamus and bone marr-ow cells. Western blot was used to assess ERαand ERβprotein expression in the right fe-moral tissue. Results Compared with the Model group, the Acu, E2, and Acu-E2 groups si-gnificantly improved BMD, bone microstructure parameters (P＜0.05), and pathological ch-anges in the femoral tissue. These treatments also reduced serum levels of bone metabolis-m markers TRACP and CTX-I, decreased FSH and GnRH levels, and increased E2 levels (P＜0.05). Furthermore, they upregulated mRNA expression of ERα, ERβ, and OPG in th-e hypothalamus and bone marrow cells while downregulating RANK and RANKL mRNA expression. Conclusion Acupuncture can effectively prevent and treat PMOP, possibly by activating the OPG/RANK/RANKL pathway, exerting a holistic regulatory effect on the hy-pothalamus-pituitary-bone axis, and enhancing the biological effects of estrogen-receptor bi-nding. |
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