| Objective To explore the effect and possible mechanism of Isopsoralen on bone remodeling in ankle fracture rats. Methods Rat model of ankle joint fracture was prepared and treated with isopsoralen and GSK-J4, Rats were divided into Control group, Model group, Isopsoralen group and Isopsoralen+GSK-J4 group. After 8 weeks of treatment, The healing of ankle fractures and ankle cartilage injuries in rats were observed; The expression of Collagen Ⅱ, matrix metalloproteinase 13 (MMP-13), Jumonji domain containing 3 (JMJD3), H3K27 trimethylation (H3K27me3), Runt related transcription factor 2 (Runx2) and bone morphogenetic protein 2 (BMP2) levels in ankle joint were detected. Results Isopsoralen could significantly reduce the joint space in rats, improved cartilage damage, increased the expression of JMJD3, Collagen Ⅱ, Runx2, BMP2 in cartilage tissue, and reduced the levels of Collagen Ⅱ, Runx2, BMP2 promoter H3K27me3 and the expression of H3K27me3 protein (P<0.05); GSK-J4 could inhibit the improvement effect of Isopsoralen on bone remodeling in rats (P<0.05). Conclusion Isopsoralen promotes bone remodeling in ankle fracture rats by enhancing JMJD3 mediated H3K27me3 demethylation. |