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| 鹿茸提取液、黄芪甲苷介导Hippo通路调控BMSCs成骨分化研究 |
| Research on antler extract and astra galoside IV mediated regulation of hippo signaling pathway in BMSCs osteogenic differentiation |
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| DOI：10.3969/j.issn.1006-7108.2026.07.002 |
| 中文关键词: 黄芪甲苷 鹿茸提取液 Taz Hippo通路 骨髓间充质干细胞 |
| 英文关键词:astragaloside IV antler extract Taz hippo pathway bone marrow mesenchymal stem cells |
| 基金项目:国家自然科学基金青年基金项目（81302879）；中国博士后科学基金第73批面上项目(2023MD734184)；辽宁省科技厅自然科学基金项目（2024-MS-121)；辽宁省教育厅重点攻关计划（LJKZZ20220103）；辽宁中医药大学“中医脏象理论及应用教育部重点实验室”开放基金项目（zyzx2201） |
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| 中文摘要: |
| 目的 观察大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells，BMSCs)在传统补肾健脾中药诱导下向成骨分化的协同调控机制。方法 按照随机化原则，实验设立5组，分别为正常组、诱导组、鹿茸组、黄芪甲苷组、黄芪甲苷+鹿茸组。茜素红染色法检测5组BMSCs矿化结节生长情况，实时荧光定量PCR（qRT-PCR）法和蛋白免疫印迹（Western blot）法分别检测Collagen Ⅰ及Hippo信号通路Lats1、Taz的mRNA与蛋白水平的表达。结果 茜素红实验结果显示，与正常组相比，用药组均观察到有矿化结节生成，提示鹿茸提取液和黄芪甲苷可在一定程度上促进BMSCs向成骨细胞转化；qRT-PCR检测结果发现，用药组Taz和Collagen Ⅰ mRNA的相对表达量相比正常组有显著上升（P＜0.01）；WB结果提示，Taz表达水平在鹿茸提取液和黄芪甲苷的影响下显著上升（P＜0.01）。结论 鹿茸提取液和黄芪甲苷能够调节Collagen Ⅰ以及Lats1、Taz表达促进BMSCs向成骨方向转化。 |
| 英文摘要: |
| Objective To delineate the coordinated regulatory network governing the osteogenic differentiation process of rat bone marrow mesenchymal stem cells (BMSCs) mediated by traditional Chinese medicinal compounds with dual kidney-tonifying and spleen-invigorating effects. Methods Based on the randomization principle, subjects were stratified into five comparative arms, including normal group, induction group, deer antler extract group, astragaloside IV group, and deer antler extract + astragaloside IV group. Mineralization capacity of BMSCs across all five groups was evaluated via Alizarin red staining. Concurrently, Quantitative real-time PCR (qRT-PCR) technology was utilized to detect transcriptional levels of collagen I along with Hippo signaling regulators Lats1 and Taz. Western blotting was conducted to examine their protein expressions. Results Comparative Alizarin red assessment showed visible mineralization deposition in the treatment groups compared to baseline controls, with the deer antler-astragaloside IV combination exhibiting the most pronounced nodule formation pattern, suggesting synergistic pro-osteogenic effects on BMSC differentiation. Comparative transcript analysis demonstrated substantially enhanced Taz and Collagen I mRNA levels across all intervention groups compared to untreated cells, with the combination group showing peak expression. This transcriptional activation was corroborated at the protein level, where Western blotting revealed dose-dependent TAZ accumulation following herbal treatment. Conclusion Deer antler extract and astragaloside IV promote osteogenic differentiation of BMSCs by modulating the expression of collagen I and key Hippo pathway-related proteins, including Lats1 and Taz. |
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