中国骨质疏松杂志

固本活血壮骨方调控BMSCs自噬治疗糖皮质激素性骨质疏松症的研究

Guben Huoxue Zhuanggu Formula regulates autophagy of BMSCs in the treatment of glucocorticoid induced osteoporosis

DOI：10.3969/j.issn.1006-7108.2026.07.005

英文关键词:GIOP GBHX PI3K-Akt signaling pathway autophagy

基金项目:陕西省教育厅重点科研计划项目（23JS006）；秦创原中医药产业创新聚集区项目（L2024-QCY-ZYYJJQ-X14）；陕西省中医体质与疾病防治重点实验室（陕科基发[2012]1号）；陕西省中医药管理局中药药效机制与物质基础重点研究室（陕中医药发[2018]32号）；陕西省自然科学基金面上项目（2025JC-YBMS-1015）

作者	单位
张惜燕* 吴雪胡勇王其鎏	陕西中医药大学,陕西咸阳 712046

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中文摘要:

目的探讨固本活血壮骨方（GBHX）治疗糖皮质激素性骨质疏松症( GIOP )的作用和机制。方法 8周龄SD大鼠随机分为空白组、模型组、西药组、中药组（n = 10）。通过血清学、骨组织病理学、Micro-CT扫描等方法检测大鼠骨质量，免疫印迹法和免疫荧光双标记法检测各组大鼠股骨组织自噬相关蛋白及信号通路相关蛋白的表达。结果实验研究结果显示，模型组股骨皮质厚度变薄，骨小梁变细，甚至断裂，脂肪空泡增多；与空白组相比，模型组大鼠B-ALP、Tb.Sp、SMI、p-Akt、p-PI3K、LC3、Beclin1上升（P＜0.05）；BV/TV、Tb.Th、BMD降低（P＜0.05）；模型组蓝色荧光均变弱，LC3、Beclin1绿色荧光表达均增加。与模型组相比，中药组和西药组骨皮质增厚，骨小梁连续性增加，数量增多、形状增粗，间距缩小，脂肪空泡减少。中药组 B-ALP、SMI、Tb.Sp、p-Akt、p-PI3K、LC3、Beclin1 下降（P＜0.05），中药组BV/TV、Tb.Th、BMD、p62升高（P＜0.05）；西药组、中药组蓝色荧光均有不同程度的增加，中药组LC3、西药组Beclin1绿色荧光表达明显减少。结论固本活血壮骨方能促进骨形成，有效改善GIOP大鼠骨质，其机制可能与PI3K/Akt信号通路介导的BMSCs细胞自噬有关。

英文摘要:

Objective To explore the effect and mechanism of the Guben Huoxue Zhuanggu Formula (GBHX) in the treatment of glucocorticoid induced osteoporosis (GIOP). Methods 8-week-old SD rats were randomly divided into a blank group, a model group, a western medicine group, and a traditional Chinese medicine group (n=10).By serological detection of B-ALP and BGP，bone tissue pathology HE staining, Micro-CT scanning to detect bone density and microstructure in rats, immunofluorescence dual labeling method and Western blot to detect the expression of autophagy related proteins Beclin1, LC3, p62 and PI3K-Akt signaling pathway related proteins in femoral tissue of rats in each group, the effect and mechanism of GBHX in improving GIOP were verified. Results The experimental research results showed that in the model group, the thickness of the femoral cortex became thinner, the bone trabeculae became thinner, and even fractured, with widened spacing and increased vacuolar fat. Compared with the blank group, the B-ALP, Tb.Sp, SMI, p-Akt, p-PI3K, LC3, and Beclin1 levels in the model group rats increased（P＜0.05）, BV/TV, Tb. Th, and BMD decreased（P＜0.05）; The blue fluorescence of the model group weakened, while the green fluorescence expression of LC3 and Beclin1 increased. Compared with the model group, the Western medicine group and the traditional Chinese medicine group showed thickening of the cortical bone, increased continuity of bone trabeculae, increased quantity and shape, reduced spacing, and decreased fat vacuoles. The levels of B-ALP, Tb.Sp, SMI, p-Akt, p-PI3K, LC3，and Beclin1 decreased in the traditional Chinese medicine group （P＜0.05）, while BV/TV, Tb. Th, BMD, and p62 increased in the traditional Chinese medicine group（P＜0.05）.The blue fluorescence of both the Western medicine group and the Chinese medicine group increased to varying degrees, while the expression of LC3 and Beclin1 green fluorescence in the Chinese medicine group and the Western medicine group decreased significantly. Conclusion GBHX can promote bone formation and effectively improve bone quality in GIOP rats. Its mechanism may be related to the autophagy of BMSCs mediated by the PI3K/Akt signaling pathway.

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