|
| 二烯丙基三硫醚通过Adcy8调节骨髓间充质干细胞成脂分化 |
| Diallyl trisulfide regulates the adipogenic differentiation of bone marrow mesenchymal stem cells through Adcy8/AKT and Adcy8/PKA pathways |
| |
| DOI：10.3969/j.issn.1006-7108.2026.08.003 |
| 中文关键词: 二烯丙基三硫醚 骨髓间充质干细胞 成脂分化 腺苷酸环化酶 8 骨质疏松 |
| 英文关键词:diallyl trisulfide bone marrow mesenchymal stem cells adipogenic differentiation adenylyl cyclase 8 osteoporosis |
| 基金项目:江苏大学医教协同创新基金（JDY2022013）；江苏大学临床医学科技发展基金（JLY2021060）；苏州市科技发展计划（医疗卫生科技创新）项目（SKYD2022055）；昆山市第一人民医院医疗卫生科技创新项目（KSKFQYLWS2023040） |
|
| 摘要点击次数: 26 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 探讨二烯丙基三硫醚（diallyl trisulfide，DATS）对骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMMSCs）成脂分化的影响及其机制。方法 （1）细胞实验：CCK8法检测DATS对BMMSCs的增殖活性影响，油红O染色检测DATS对BMMSCs的成脂分化影响，荧光定量PCR检测成脂基因的mRNA表达量，高通量测序BMMSCs成脂分化后的mRNA，慢病毒转染敲除BMMSCs的Adcy8基因，Western blot检测DATS对AKT、PKA等蛋白的影响；（2）动物实验：8周龄SD大鼠分为SHAM组、OVX组、OVX+DATS（40 mg/kg）组，12周后DXA测量骨密度和身体成分，Micro-CT扫描分析骨微结构和参数变化，H&E染色观察组织学改变。结果 （1）DATS对BMMSCs增殖活性无抑制作用，可抑制BMMSCs的成脂分化，DATS可促进BMMSCs的Adcy8表达，并通过Adcy8影响BMMSCs的成脂分化，DATS抑制AKT磷酸化，同时也促进了PKA的蛋白表达量；（2）与OVX组比较，DATS干预可明显减少大鼠体重、体脂和体脂百分比，并减少骨髓腔脂肪细胞数量。结论 DATS可能通过Adcy8/AKT、Adcy8/PKA调节BMMSCs的成脂分化，从而减少骨髓腔脂肪堆积。 |
| 英文摘要: |
| Objective To investigate the effects of diallyl trisulfide (DATS) on adipogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs) and its underlying mechanisms. Methods (1) In vitro experiments: cell viability was assessed using the CCK-8 assay. Adipogenic differentiation was evaluated with Oil Red O staining. mRNA expression of adipogenic genes was quantified via quantitative real-time PCR (RT-qPCR). Transcriptome sequencing (RNA-seq) was performed in BMMSCs after adipogenic differentiation. Adcy8 was knocked down in BMMSCs using lentiviral transduction. Protein expressions of AKT and PKA were analyzed using Western blotting. (2) In vivo experiments: 8-week-old SD rats were divided into three groups: SHAM, OVX, and OVX + DATS (40 mg/kg). After 12 weeks of intervention, bone mineral density (BMD) and body composition were measured with dual-energy X-ray absorptiometry (DXA). Bone microstructure and parameters were analyzed with micro-computed tomography (micro-CT). Histological changes were observed using hematoxylin and eosin (H&E) staining. Results (1) DATS did not inhibit BMMSC proliferation. DATS suppressed adipogenic differentiation of BMMSCs. DATS promoted Adcy8 expression and regulated adipogenic differentiation through Adcy8-dependent mechanisms. DATS inhibited AKT phosphorylation and increased PKA protein expression. (2) Compared with the OVX group, DATS intervention significantly reduced body weight, fat mass, and body fat percentage (P<0.05), and decreased bone marrow adipocyte accumulation. Conclusion DATS may regulate adipogenic differentiation of BMMSCs through the Adcy8/AKT and Adcy8/PKA pathways, thereby reducing bone marrow adiposity. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |